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Action of natural phytosanitary products on Bacillus thuringiensis subsp. kurstaki S-1905

Lozano, E.R., Neves, P.M.O.J., Alves, L.F.A., Potrich, M., Vilas-Bôas, G.F.L.T., Monnerat, R.G.
Bulletin of entomological research 2018 v.108 no.2 pp. 223-231
Anticarsia gemmatalis, Bacillus thuringiensis subsp. kurstaki, Erebidae, additive effect, agar, agar gel electrophoresis, bacteria, bacterial toxins, crystal proteins, crystals, diet, mortality, pathogens, protein degradation, scanning electron microscopy, spores
The objective of this study was to evaluate the effects of natural phytosanitary products (NPs) on spores and crystals of Bacillus thuringiensis subsp. kurstaki S-1905 (Btk S-1905). For the spore assay, NPs and bacteria were applied in combination and individually. For the combined application, Btk S-1905 + NP mixtures were inoculated on nutrient agar (NA), and for the separate applications, the NPs were spread on NA plates, which were later inoculated with the pathogen. The number of colony-forming units (CFU) per milliliter was quantified after 18 h of incubation. For the crystal protein degradation assay, the Btk S-1905 + NP mixtures were added to the diet of Anticarsia gemmatalis (Lepidoptera: Erebidae), and mortality was evaluated at the following time points: 12, 24, 48, and 72 h. Scanning electron microscopy and agarose gel electrophoresis were carried out. Biogermex and Ecolife® reduced the CFU ml⁻¹ in both combined and separate applications. Biogermex, Ecolife®, and Planta Clean were antagonistic to the action of bacterial toxins, and no product affected the morphology or resulted in the degradation of the crystal proteins. The remaining products evaluated did not reduce the CFU ml⁻¹ and had additive effect when combined with the crystal toxin.