U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

Tart Cherry Reduces Inflammation in Adipose Tissue of Zucker Fatty Rats and Cultured 3T3-L1 Adipocytes

Author:
Shasika Jayarathne, April J. Stull, Alexandra Miranda, Shane Scoggin, Kate Claycombe-Larson, Jung Han Kim, Naima Moustaid-Moussa
Source:
Nutrients 2018 v.10 no.11 pp. -
ISSN:
2072-6643
Subject:
Prunus cerasus, adipocytes, adipokines, adipose tissue, anti-inflammatory activity, antioxidants, arginase, chemokine CCL2, diet, epididymis, fatty-acid synthase, gene expression, health status, inducible nitric oxide synthase, inflammation, interleukin-1beta, interleukin-6, lipid peroxidation, lipopolysaccharides, messenger RNA, models, obesity, peroxisome proliferator-activated receptor alpha, phosphorylation, protein content, rats, secretion, transcription factor NF-kappa B, tumor necrosis factor-alpha
Abstract:
Obesity increases adipose tissue inflammation and secretion of pro-inflammatory adipokines, which have systemic effects on the organism&rsquo;s health status. Our objective was to dissect mechanisms of anti-inflammatory effects of tart cherry (TC) in adipose tissue of Zucker fatty rats, and cultured 3T3-L1 adipocytes. Rats were fed either a control diet, or 4% TC powder diets for eight weeks. Body and epididymal fat pad weights were not significantly different between control and TC groups. However, rats fed the TC diet had significantly reduced adipose tissue inflammation (p < 0.05), as determined by reduced mRNA levels of pro-inflammatory markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF&alpha;), interleukin-1beta (IL-1&beta;), monocyte chemoattractant protein 1 (MCP-1), inducible nitric oxide synthase (iNOS), and CD-11b, and increased mRNA levels of type-1 arginase (Arg-1) anti-inflammatory marker. Consistent with these in vivo results, TC significantly decreased expression of IL-6 mRNA and protein levels in lipopolysaccharide (LPS) stimulated adipocytes compared to those stimulated with LPS, but no TC. Moreover, both in vivo (rat adipose tissue) and in vitro (3T3-L1 adipocytes), phosphorylation of p65-NF-&kappa;B subunit was significantly reduced by TC. Additionally, TC decreased mRNA expression of fatty acid synthase (FASN), and increased expression of peroxisome proliferator-activated receptor alpha (PPAR&alpha;), master regulator of lipid oxidation, and anti-oxidant markers nuclear factor erythroid-derived 2-related factor (NRFs) in both models. In conclusion, our findings indicate that TC downregulates inflammation in part via the nuclear factor kappa B (NF-&kappa;B) pathway in adipose tissue. Thus, TC may serve as a potential intervention to reduce obesity-associated inflammation.
Agid:
6506204
Handle:
10113/6506204