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Fatty Acid Desaturase Involvement in Non-Alcoholic Fatty Liver Disease Rat Models: Oxidative Stress Versus Metalloproteinases

Author:
Palladini, Giuseppina, Di Pasqua, Laura G., Berardo, Clarissa, Siciliano, Veronica, Richelmi, Plinio, Mannucci, Barbara, Croce, Anna Cleta, Rizzo, Vittoria, Perlini, Stefano, Vairetti, Mariapia, Ferrigno, Andrea
Source:
Nutrients 2019 v.11 no.4
ISSN:
2072-6643
Subject:
animal models, blood serum, diet, enzyme activity, fatty acid composition, fatty liver, gas chromatography-mass spectrometry, laboratory animals, liver, males, metalloproteinases, oxidative stress, protein synthesis, rats, stearoyl-CoA desaturase, therapeutics, thiobarbituric acid-reactive substances, tumor necrosis factor-alpha
Abstract:
We investigated changes in fatty acid desaturases, D5D, D6D, D9-16D and D9-18D, and their relationship with oxidative stress, matrix metalloproteinases (MMPs) and serum TNF-alpha in two rat models of non-alcoholic fatty liver disease NAFLD. Eight-week-old male Wistar rats fed for 3 weeks with methionine-choline–deficient (MCD) diet and eleven-week-old Obese male Zucker rats were used. Serum levels of hepatic enzymes and TNF-alpha were quantified. Hepatic oxidative stress (ROS, TBARS and GSH content) and MMP-2 and MMP-9 (protein expression and activity) were evaluated. Liver fatty acid profiling, performed by GC-MS, was used for the quantification of desaturase activities. Higher D5D and D9-16D were found in Obese Zucker rats as well as an increase in D9-18D in MCD rats. D6D was found only in MCD rats. A negative correlation between D5D and D9-16D versus TBARS, ROS and TNF-alpha and a positive correlation with GSH were shown in fatty livers besides a positive correlation between D9-18D versus TBARS, ROS and TNF-alpha and a negative correlation with GSH. A positive correlation between D5D or D9-16D or D9-18D versus protein expression and the activity of MMP-2 were found. NAFLD animal models showed comparable serum enzymes. These results reinforce and extend findings on the identification of therapeutic targets able to counteract NAFLD disorder.
Agid:
6507724