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A high-throughput, bead-based, antigen-specific assay to assess the ability of antibodies to induce complement activation
- Fischinger, Stephanie, Fallon, Jonathan K., Michell, Ashlin R., Broge, Thomas, Suscovich, Todd J., Streeck, Hendrik, Alter, Galit
- Journal of immunological methods 2019
- antibodies, antigen-antibody complex, antigens, complement, innate immunity, pathogens
- The complement system plays a critical role in innate immune defense against pathogens, both via non-specific direct pathogen recognition and killing or via antigen-specific indirect recruitment by complement fixing antibodies. While various assays for measuring complement activation have been developed, few provide a high-throughput, sample-sparing approach to interrogate the qualitative differences in the ability of antibodies to drive complement activation. Here we present a high-throughput, sample-sparing, bead-based assay to evaluate antigen-specific antibody-dependent complement activation against nearly any antigen. Optimization of buffer composition, kinetics of immune complex formation, as well as complement source all contribute critically to the development of a robust, highly flexible and high-throughput approach to analyze antibody-dependent complement deposition (ADCD). Thus, the optimized bead-based, antigen-specific assay represents a simple, highly adaptable platform to profile antibody-dependent complement activation across pathogens and diseases.