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An l-glutaminase enzyme reactor based on porous bamboo sticks and its application in enzyme inhibitors screening

Qiao, Juan, Zhao, Liping, Liu, Lili, Qi, Li
Talanta 2019 v.205 pp. 120126
bamboos, batch systems, chemical bonding, electrochromatography, enzymatic hydrolysis, enzyme inhibitors, glutaminase, glutamine, glutaraldehyde, hydrolysis, ligands, micropores, pharmaceutical industry, screening
Inspired by the porous and fibrous structure of commercially available bamboo, herein we created an l-glutaminase enzyme reactor based on bamboo sticks. The enzyme was immobilized onto the bamboo sticks through a glutaraldehyde modification to achieve covalent bonding. The enzymatic hydrolysis efficiency of the prepared l-glutaminase@bamboo sticks based porous enzyme reactor was evaluated by chiral ligand exchange capillary electrochromatography using l-glutamine as the substrate. l-glutaminase@bamboo exhibited improved enzymatic hydrolysis performances, including high hydrolysis efficiency (maximum rate Vmax: two fold higher than the free enzyme), prolonged stability (14 days) and good reusability. l-Glutaminase@bamboo sticks also expanded application capability in pharmaceutical industry in enzyme inhibitor screening. These excellent properties could be attributed to the micropores of bamboo sticks, which led to the fast enzymatic kinetics. The results suggest that the pores of bamboo sticks played an important role in the proposed enzyme reactor during the hydrolysis of l-glutamine and l-glutaminase inhibitor screening.