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Development of three-dimensional bacterial cellulose/chitosan scaffolds: Analysis of cell-scaffold interaction for potential application in the diagnosis of ovarian cancer

Ul-Islam, Mazhar, Subhan, Fazli, Islam, Salman Ul, Khan, Shaukat, Shah, Nasrullah, Manan, Sehrish, Ullah, Muhammad Wajid, Yang, Guang
International journal of biological macromolecules 2019 v.137 pp. 1050-1059
biocompatibility, biocompatible materials, cellulose, chitosan, confocal microscopy, cytoskeleton, gene expression, geometry, histology, human cell lines, neoplasm cells, ovarian neoplasms, receptors, reverse transcriptase polymerase chain reaction, scanning electron microscopy, staining, viability
Bacterial cellulose (BC) has emerged as a biomaterial for diverse biomedical applications owing to its unique structural, physico-chemical, mechanical, and biological features. Its porous geometry and three-dimensional fibrous structure allow the impregnation of various materials into its matrix. The current study was aimed to fabricate 3D scaffolds of bacterial cellulose and chitosan (BC-Chi) through a one-step ex situ solution impregnation strategy and analyze the scaffold interaction with the ovarian cancer cell lines (A2780). Field emission scanning electron microscopy (FE-SEM) showed successful impregnation of chitosan into the BC matrix. Phase-contrast and confocal microscopy analyses revealed that human ovarian cancer cell lines (A2780) were adhered not only to the surface but deeply infiltrated into the matrix of BC-Chi scaffold. WST-1 assay, histology analysis, and cytoskeleton and nuclear staining showed high viability, proliferation, and infiltration of A2780 cell lines into the scaffold. The RT-PCR analysis revealed a decreased mRNA level of Notch receptors, indicating a strong cell-scaffold interaction. The improved biocompatibility, non-toxicity, and 3D structure of fabricated BC-Chi scaffold justify its potential applications diagnosis of ovarian cancer in vivo.