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High-pressure ultrasonic-assisted extraction of polysaccharides from Hovenia dulcis: Extraction, structure, antioxidant activity and hypoglycemic

Author:
Yang, Bing, Wu, Qunjun, Luo, Yuxin, Yang, Qiong, Wei, Xunyu, Kan, Jianquan
Source:
International journal of biological macromolecules 2019 v.137 pp. 676-687
ISSN:
0141-8130
Subject:
2,2-diphenyl-1-picrylhydrazyl, Hovenia dulcis, X-ray diffraction, antioxidant activity, arabinose, chelation, galactose, galacturonic acid, glucose, glycemic effect, hydroxyl radicals, hypoglycemic agents, in vivo studies, iron, mannose, molecular weight, polysaccharides, response surface methodology, rhamnose, ultrasonics
Abstract:
In this study, high-pressure ultrasonic-assisted extraction (HUE) technology was used for polysaccharide extraction from Hovenia dulcis (HDPS). A maximal extraction yield (11.81 ± 0.26%) was obtained using the response surface method (RSM) with optimized HUE conditions (Ultrasound power 330 W, 68 °C, and 22 min). Results showed that HDPS mainly contained neutral sugar (47.81 ± 1.56%), uronic acid (12.52 ± 0.15%), and protein (3.64 ± 0.24%), specifically. HDPS was comprised of mannose, rhamnose, galacturonic acid, glucose, galactose and arabinose in a molar ratio of 3.20%, 20.40%, 4.81%, 11.14%, 27.37% and 33.08%, respectively. And with an average molecular weight of 185.22 kDa for its major component (accounting for 90.13% for HDPS). The result obtained from the X-ray diffraction pattern confirmed a semi-crystalline nature of the sample. HUE-HDPS suggested typical shear thinning behavior and non-Newtonian properties. Additionally, HUE-HDPS exhibited better chemical antioxidant activities in DPPH, ABTS, hydroxyl radical scavenging and Fe2+ chelation activity. The in-vitro and in-vivo experiments indicated that HUE-HDPS had obviously hypoglycemic effects. Accordingly, the polysaccharides from Hovenia dulcis might serve as a natural antioxidant and hypoglycemic agent.
Agid:
6509738