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A novel fatty alkene from marine bacteria: A thermo stable biosurfactant and its applications

Author:
Ram, Hari, Kumar Sahu, Amit, Said, Madhukar S., Banpurkar, Arun G., Gajbhiye, Jayant M., Dastager, Syed G.
Source:
Journal of hazardous materials 2019 v.380 pp. 120868
ISSN:
0304-3894
Subject:
Bacillus (bacteria), Fourier transform infrared spectroscopy, aquatic bacteria, bioreactors, bioremediation, biosurfactants, contact angle, droplets, emulsifying, high performance liquid chromatography, mass spectrometry, nuclear magnetic resonance spectroscopy, nucleotide sequences, oils, pH, pesticides, ribosomal RNA, sand, sodium chloride, surface tension, tap water, thermal stability, yeast extract
Abstract:
In this study, a novel thermo stable biosurfactants, 1-Pentanonacontene (C95H190) a fatty alkene and 3-Hydroxy-16-methylheptadecanoic acid (C18H36O3) were isolated from a marine isolate SGD-AC-13. Biosurfactants were produced using 1% yeast extract in tap water as production medium at 24 h in flask and 12 h in bioreactor. Using 16S rRNA gene sequence (1515 bp) and BCL card (bioMérieux VITEK®), strain was identified as Bacillus sp. Crude biosurfactant reduced the surface tension of distilled water to 31.32 ± 0.93 mN/m with CMC value of 0.3 mg/ml. Cell free supernatant showed excellent emulsification and oil displacement activity with stability up to 160 °C, pH 6–12 and 50 g/L NaCl conc. Biosurfactants were characterized using FTIR, TLC, HPLC LC–MS and NMR spectroscopy.Cell free supernatant reduced the contact angle of distilled water droplet from 117° to 52.28° and of 2% pesticide from 78.77° to 73.42° while 750 μg/ml of crude biosurfactant reduced from 66.06° to 56.33° for 2% pesticide and recovered 35% ULO and 12% HWCO from the contaminated sand. To our best of knowledge, this is the first report of thermo stable fatty alkene as a biosurfactant and is structurally different from previously reported, with having potential application in agriculture, oil recovery and bioremediation.
Agid:
6510114