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Effects of tetracycline on growth, oxidative stress response, and metabolite pattern of ryegrass

Author:
Han, Tao, Liang, Yueping, Wu, Zhineng, Zhang, Li, Liu, Zhenwei, Li, Qingfei, Chen, Xuejin, Guo, Weili, Jiang, Lina, Pan, Feifei, Ge, Shidong, Mi, Zhaorong, Liu, Zunchun, Huang, Hua, Li, Xinzheng, Zhou, Junguo, Li, Yang, Wang, Jialiang, Zhang, Zhen, Tang, Yingying, Yang, Linru, Wu, Mengdan
Source:
Journal of hazardous materials 2019 pp. 120885
ISSN:
0304-3894
Subject:
Lolium, aconitic acid, alanine, aminoacyl transfer RNA, asparagine, aspartic acid, biochemical pathways, biosynthesis, carotenoids, chlorophyll, galactose, gluconic acid, glucose, glutamic acid, lactose, mannitol, membrane potential, metabolites, mitochondrial membrane, nitrogen metabolism, oxidative stress, permeability, phenylalanine, reactive oxygen species, roots, seedlings, serine, shikimic acid, stress response, tetracycline
Abstract:
Tetracycline is an antibiotic that frequently contaminates the environment. In this study, the growth and metabolites of ryegrass seedlings treated with tetracycline (0, 1, 10 or 100 mg/L) for 5 days were investigated. The results showed that the growth of ryegrass and the concentrations of carotenoid and chlorophyll decreased as the tetracycline concentration increased. Tetracycline increased the production of reactive oxygen species (ROS) and cell permeability and triggered mitochondrial membrane potential loss in the roots of ryegrass. The metabolic profiles of ryegrass differed between the control and tetracycline-treated groups. The contents of glucose, shikimic acid, aconitic acid, serine, lactose, phenylalanine, mannitol, galactose, gluconic acid, asparagine, and glucopyranose were positively correlated with root length and had high variable importance projection values. These compounds may have crucial functions in root extension. Tetracycline also affected aminoacyl-tRNA biosynthesis, nitrogen metabolism, and alanine, aspartate and glutamate metabolism in the roots. Tetracycline may affect root extension by regulating the synthesis/degradation of these metabolites or the activity of their biosynthetic pathways. These results provide an insight into the stress response of ryegrass to tetracycline.
Agid:
6510119