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Saccharification of kans grass biomass by a novel fractional hydrolysis method followed by co-culture fermentation for bioethanol production
- Mishra, Archana, Ghosh, Sanjoy
- Renewable energy 2020 v.146 pp. 750-759
- Scheffersomyces, Zymomonas mobilis, biomass, coculture, ethanol, ethanol production, fermentation, grasses, hydrolysates, hydrolysis, lignocellulose, pentoses, reducing sugars, renewable energy sources, saccharification, sulfuric acid, toxicity
- Present work is focused mainly on recovering maximum amount of soluble pentose and hexose sugars separately, direct from the kans grass (lignocellulosic biomass) by a novel fractional hydrolysis process (pretreatment + hydrolysis + detoxification) with minimum toxic products generation. Effect of biomass loading and preheating time on the fractional hydrolysis process was studied along with various strategies up to 30% H2SO4 (v/v) to reduce the number of stages for acid use minimisation. 84.88% of total reducing sugar present in the kans grass biomass was extracted as separate pentose and hexose sugar fractions with negligible toxics. Zymomonas mobilis and Scheffersomyces shehatae were used during co-culture fermentation; 92.13% of the sugar present in xylose-rich fraction (initial sugar: 21.87 g/L) and 96.32% of glucose-rich fraction (initial sugar: 40.32 g/L) were utilised to produce 25.0 g/L ethanol from the kans grass biomass hydrolysate; thereby achieving 78.6% of the maximum theoretical ethanol production with an average yield of 0.435.