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Three-dimensional fine structure of feeder organelle in Cryptosporidium parvum

Author:
Bochimoto, Hiroki, Kondoh, Daisuke, Ishihara, Yo, Kabir, Mohammad Hazzaz Bin, Kato, Kentaro
Source:
Parasitology international 2019 v.73 pp. 101958
ISSN:
1383-5769
Subject:
Cryptosporidium parvum, epithelial cells, ileum, mice, nutrient uptake, organelles, proteins, scanning electron microscopy, transmission electron microscopy, ultrastructure
Abstract:
Feeder organelles of Cryptosporidium are the convoluted structures located at the host-parasite interface that uptake of nutrients from host cells. Although the ultrastructure of feeder organelles has been summarized as being highly invaginated structure, the three-dimensional form remains uncertain. Osmium-maceration scanning electron microscopy (OS-SEM) allows visualization of the three-dimensional ultrastructure after removing soluble proteins. Here, we assessed C. parvum attached to mouse ileal epithelial cells using transmission electron microscopy (TEM) and OS-SEM. Feeder organelles visualized by TEM as aggregated structures of concentrically-, vertically- and randomly-lined bars comprised a complex reticulated network of stacked flat bursiform, ring-shaped bursiform and reticulated tubular membranes on OS-SEM. These findings suggested that the feeder organelles are more complex than was previously thought.
Agid:
6510877