U.S. flag

An official website of the United States government

PubAg

Main content area

Detection and molecular identification of Hepatozoon canis and Babesia vogeli from domestic dogs in Palestine

Author:
AZMI, KIFAYA, AL-JAWABREH, AMER, NASEREDDIN, ABEDELMAJEED, ABDELKADER, AHMAD, ZAID, TAHER, EREQAT, SUHEIR, SAWALHA, SAMER S., BANETH, GAD, ABDEEN, ZIAD
Source:
Parasitology 2017 v.144 no.5 pp. 613-621
ISSN:
1469-8161
Subject:
Babesia canis subsp. vogeli, DNA, Hepatozoon canis, Hepatozoon felis, Rhipicephalus sanguineus, blood sampling, disease control, dogs, genes, hemoparasites, hosts, nucleotide sequences, oligodeoxyribonucleotides, pathogens, polymerase chain reaction, ribosomal RNA, ticks, Palestine, West Bank
Abstract:
Dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. This study aimed to observe tick borne pathogens in dogs from Palestine including Hepatozoon canis and Babesia species. The prevalence of both H. canis and Babesia species infections in apparently healthy dogs, from ten districts of the West Bank was surveyed. DNA was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). A primer set that amplifies a partial sequence of the Babesia and Hepatozoon 18S rRNA gene was used for PCR and the DNA sequences of the PCR products of all samples were determined. Twenty-nine (8·0%) of the dogs were found infected including 20 with H. canis (5·5%), seven with Babesia vogeli (1·9%) and two with undefined Babesia spp. (0·6%). Twelve Rhipicephalus sanguineus s.l ticks were pathogen-positive, including ten with H. canis (4·7%), one with B. vogeli (0·5%), and one with Hepatozoon felis (0·5%). The results indicated that a wide range of tick borne pathogens is circulating in the canine population in the surveyed region. This study is the first report on the prevalence of H. canis, B. vogeli and Babesia spp. in dogs in Palestine and its results will assist in the management of diseases associated with these blood parasites.
Agid:
6511970