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Identification of the LEA family members from Caragana korshinskii (Fabaceae) and functional characterization of CkLEA2-3 in response to abiotic stress in Arabidopsis

Yu, Xiumin, Yue, Wenran, Yang, Qi, Zhang, Yanna, Han, Xiaomin, Yang, Feiyun, Wang, Ruigang, Li, Guojing
Revista brasileira de botânica 2019 v.42 no.2 pp. 227-238
Arabidopsis thaliana, Caragana korshinskii, abscisic acid, cDNA libraries, embryogenesis, genes, plant adaptation, proteins, quantitative polymerase chain reaction, salt stress, seed germination, seedlings, stress tolerance, tissues, transcriptomics, water stress
Plants are exposed to different abiotic stresses that affect growth, development, and productivity. Previous studies have shown that late embryogenesis abundant (LEA) proteins play important roles in plant adaptation to abiotic stresses. However, reports that demonstrate their biological functions, especially in Caragana korshinskii Kom., are still very limited. In this study, 26 LEA genes were identified from dehydration-treated suppressive subtractive hybridizations cDNA library and transcriptome sequencing data of C. korshinskii, and were classified into seven groups according to their structural features. Quantitative real-time PCR analysis revealed that the CkLEAs were induced by abscisic acid (ABA) and diverse abiotic stresses and widely expressed in various tissues. Moreover, overexpression of CkLEA2-3 in Arabidopsis thaliana L. Heynh resulted in enhanced tolerance to ABA treatment, and osmotic and salt stresses during seed germination. CkLEA2-3 overexpression lines also exhibited resistance to drought stress during seedling development. Taken together, our results indicate that CkLEA2-3 plays positive roles in conferring abiotic stress tolerance in Arabidopsis.