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Preparative column chromatography of four groups of isoflavones from soybean cake
- Kao, T. H., Lu, Y. F., Chen, B. H.
- European food research & technology 2005 v.221 no.3-4 pp. 459-465
- adsorbents, glucosides, high performance liquid chromatography, isoflavones, silica gel, soybean cake
- Various adsorbents and solvent systems were compared with respect to the separation efficiency of four classes of isoflavones, malonylglucosides, acetylglucosides, glucosides and aglycones, in soybean cake by preparative column chromatography. The various fractions of isoflavones collected from column were monitored by HPLC. Soybean cake was found to contain malonylglucosides (803.70 μg/ml), acetylglucosides (85.57 μg/ml), glucosides (728.25 μg/ml) and aglycones (53.02 μg/ml). Both malonylglucosides and glucosides were separated using Diaion HP-20 as adsorbent and a solvent system of deionized water-ethanol with a ratio of 85:15 (v/v) and 73:27 (v/v), respectively. Whereas, both acetylglucosides and aglycones were separated using silica gel as adsorbent and a solvent system of n-hexane-isopropanol-ethanol (8:9:1, v/v/v). A total amount of 244.26, 21.25, 179.04 and 11.88 μg/mg were obtained for malonylglucosides, acetylglucosides, glucosides and aglycones, respectively.