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Isolation and characterization of microsatellite markers for the red alga Porphyra umbilicalis

Author:
Varela-Álvarez, Elena, Balau, Ana C., Paulino, Cristina, Berecibar, Estibaliz, Pearson, Gareth A., Serrão, Ester A.
Source:
Plant genetic resources 2018 v.16 no.4 pp. 390-393
ISSN:
1479-263X
Subject:
DNA libraries, Porphyra, Pyropia, algae, genetic markers, loci, microsatellite repeats, null alleles, population genetics, population structure
Abstract:
The red algal genus Porphyra is sister to the genus Pyropia, the single most valuable marine crop in the Orient. We developed microsatellite loci for the red alga Porphyra umbilicalis, a widespread species in the Northern Atlantic. Enriched DNA libraries were constructed and 68 loci were screened for amplification and polymorphism. Seven polymorphic microsatellite markers were isolated using 44 individuals collected from four natural populations. The number of alleles per locus ranged from two to 12. Null alleles were detected in three loci. Among the markers reported, we tested also cross-amplification with two other Porphyra spp. These polymorphic microsatellite markers should be useful for investigating population genetic structure of P. umbilicalis in the North East Atlantic.
Agid:
6523913