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1Mx2.1, a novel high-molecular-weight glutenin subunit from Aegilops comosa and its relationship to high-quality dough

Author:
Du, Xuye, Xia, Biya, He, Fang, Ren, Mingjian
Source:
Plant genetic resources 2019 v.17 no.4 pp. 379-381
ISSN:
1479-263X
Subject:
Aegilops comosa, Triticum, amino acid sequences, chromosomes, cultivars, cysteine, dough, dough quality, endosperm, genes, glutenins, mixing, processing quality, wheat
Abstract:
High-molecular-weight glutenin subunit (HMW-GS) of endosperm is mainly correlated with dough quality of bread wheat. In wheat cultivars, the HMW-GS genes with good processing quality are limited. However, there are an amount of excellent HMW-GS genes presenting in wheat-related species. In this work, two novel HMW-GS genes located on 1 M chromosome from Aegilops comosa have been cloned, designated as 1Mx2.1 and 1My12.1, respectively. The molecular structure of 1Mx2.1 and 1My12.1 showed high similarity with the published HMW-GS, but containing unique structures. 1Mx2.1 contained an extra cysteine residue in the repetitive domain, and 1My12.1 lost the conservative cysteine residue in the C-terminal domain. In vitro mixing test has indicated that 1Mx2.1 contributes excellent dough quality. The Ae. comosa can be used as an important genetic resource for wheat quality improvement.
Agid:
6523984