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Characterization of microsatellite loci from two-spotted octopus Octopus bimaculatus Verrill 1883 from pyrosequencing reads

Author:
Domínguez-Contreras, J. F., Munguía-Vega, A., Ceballos-Vázquez, B. P., Arellano-Martínez, M., Culver, M.
Source:
Conservation genetics resources 2014 v.6 no.2 pp. 465-468
ISSN:
1877-7252
Subject:
Octopodidae, alleles, loci, microsatellite repeats, sequence analysis
Abstract:
We characterized 22 novel microsatellite loci in the two-spotted octopus Octopus bimaculatus using 454 pyrosequencing reads. All loci were polymorphic and will be used in studies of marine connectivity aimed at increasing sustainability of the resource. The mean number alleles per locus was 13.09 (range 7–19) and observed heterozygosities ranged from 0.50 to 1.00. Four loci pairs were linked and three deviated from Hardy–Weinberg equilibrium. Eighteen and 12 loci were polymorphic in Octopus bimaculoides and Octopus hubbsorum, respectively.
Agid:
652828