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Characterisation of polymorphic microsatellite loci for the bryozoan Fredericella sultana, the primary host of the causative agent of salmonid proliferative kidney disease

Author:
Filippenko, Dmitry, Hartikainen, Hanna, Okamura, Beth, Vasemägi, Anti
Source:
Conservation genetics resources 2014 v.6 no.2 pp. 481-482
ISSN:
1877-7252
Subject:
Bryozoa, DNA, DNA libraries, Salmonidae, fish, freshwater, genome, kidneys, loci, microsatellite repeats, parasites
Abstract:
The freshwater bryozoan Fredericella sultana is the most common primary host for the myxozoan parasite Tetracapsuloides bryosalmonae, causing proliferative kidney disease (PKD) in salmonid fish. An Illumina next-generation sequencing library containing genomic DNA from both T. bryosalmonae and F. sultana yielded 10,653 microsatellite motifs. Twenty-four loci were tested in both species and 12 novel polymorphic tri- and tetranucleotide microsatellite markers were developed for F. sultana. These markers provide a valuable resource for population genetic studies of F. sultana, whilst the apparent lack of microsatellites in the PKD agent suggests a low frequency of tri- and tetranucleotide repeats in the T. bryosalmonae genome.
Agid:
652833