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Development and characterization of eighteen microsatellites for endangered fish Huso dauricus

Author:
Cuiyun, Lu, Qianqian, Yin, Lei, Cheng, Ying, Gu, Chao, Li, Xiaowen, Sun
Source:
Conservation genetics resources 2014 v.6 no.2 pp. 361-365
ISSN:
1877-7252
Subject:
Huso, alleles, fish, genetic techniques and protocols, genomic libraries, heterozygosity, loci, microsatellite repeats, overfishing, pollution, watersheds
Abstract:
The Kaluga (Huso dauricus) is one of two species of Acipenseriformes endemic to the Amur River basin. Due to overfishing and environmental pollution, this species has been assessed as critically endangered by IUCN. In this study, eighteen polymorphic microsatellite markers were developed from an enriched genomic library for H. dauricus and characterized in thirty-one individuals. The number of alleles per locus ranged from two to ten with an average of 5.056. The observed heterozygosity and the expected heterozygosity varied from 0.238 to 1.000 (mean = 0.780) and from 0.209 to 0.855 (mean = 0.661), respectively. These polymorphic loci will be valuable tools for the population genetic analysis and conservation of H. dauricus.
Agid:
652859