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Protective Effect of Schisandra chinensis Polysaccharides Against the Immunological Liver Injury in Mice Based on Nrf2/ARE and TLR4/NF-κB Signaling Pathway

Yingying Shan, Bin Jiang, Jiahui Yu, Jiaye Wang, Xiaoli Wang, He Li, Chunmei Wang, Jianguang Chen, Jinghui Sun
Journal of medicinal food 2019 v.22 no.9 pp. 885-895
Schisandra chinensis, Toll-like receptor 4, alanine transaminase, alcohols, antioxidant activity, aspartate transaminase, blood serum, carbon tetrachloride, concanavalin A, gene expression regulation, genes, glutathione, heme oxygenase (biliverdin-producing), hepatoprotective effect, high fat diet, interferon-gamma, interleukin-1beta, interleukin-6, liver, malondialdehyde, mice, neoplasms, nitric oxide, oxidative stress, polysaccharides, signal transduction, superoxide dismutase, transcription factor NF-kappa B, tumor necrosis factor-alpha
We have previously demonstrated the hepatoprotective effect of Schisandra chinensis polysaccharides (SCP) against the liver injury induced by alcohol, high-fat diet, and carbon tetrachloride in mice. In this study, we investigated the effect of SCP against the immunological liver injury induced by concanavalin A (Con A) in mice. The results showed that SCP could significantly reduce the level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the serum of mice with immunological liver injury. SCP could significantly decrease the content of malondialdehyde (MDA) and nitric oxide (NO) and increase the activity of superoxide dismutase (SOD) and glutathione (GSH) in the liver tissue. SCP could significantly increase the number of CD4⁺ and decrease the number of CD8⁺ in the peripheral blood, and elevate the ratio of CD4⁺/CD8⁺. SCP could significantly downregulate the expression of Kelch-like ECH-associated protein 1 (Keap1) and upregulate the expression of nuclear factor-erythroid 2-related factor2 (Nrf2) and downstream gene heme oxygenase-1 (HO-1), and downregulate the expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response gene 88 (MyD88), and nuclear factor-kappa B (NF-κB) proteins. This study indicates that SCP can reduce the release of a large number of inflammatory factors to inhibit the oxidative stress in mice with the immunological liver injury induced by Con A, and its mechanism is closely related to the regulation of Nrf2/antioxidant response element and TLR4/NF-κB signaling pathways.