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Hemocompatible hemoadsorbent for effective removal of protein-bound toxin in serum

Li, Qingsi, Yang, Jing, Cai, Nana, Zhang, Jiamin, Xu, Tong, Zhao, Weiqiang, Guo, Hongshuang, Zhu, Yingnan, Zhang, Lei
Journal of colloid and interface science 2019 v.555 pp. 145-156
adsorbents, adsorption, bilirubin, biocompatibility, bovine serum albumin, coatings, drugs, encapsulation, fetal bovine serum, hemolysis, hydrogels, liver failure, microparticles, molecular weight, patients, permeability, phenolsulfonphthalein, poisoning, polycarboxybetaines, polyhydroxyethyl methacrylates, polystyrenes, toxins, zwitterions
Resin hemoperfusion is a life-saving treatment for drug intoxication or hepatic failure of patients. However, current resin adsorbents exhibit a limited hemocompatibility or low adsorption efficiency, representing a major roadblock to successful clinical applications. In this work, we developed a hemocompatible and effective hemoadsorbent based on polystyrene resin (H103) microparticles encapsulated in anti-biofouling zwitterionic poly(carboxybetaine) (PCB) hydrogels. Apart from a strong mechanical stability, this PCB-based adsorbent (PCB-H103) exhibited excellent hemocompatibility (hemolysis ratio was ∼0.64%), which was attributed to the anti-biofouling property of PCB hydrogel. In addition, it can efficiently adsorb both small and middle molecular weight molecules in phosphate-buffered saline, and the efficiencies were significantly higher than poly(ethylene glycol) methacrylate-based and poly(2-hydroxyethyl methacrylate)-based adsorbent counterparts, indicating the favorable permeability of PCB hydrogel coating. More importantly, PCB-H103 could effectively remove protein-bound toxins including phenol red and bilirubin in bovine serum albumin solution or even in 100% fetal bovine serum (FBS). In 100% FBS, the adsorption capacity of PCB-H103 towards bilirubin was 8.3 times higher than that of pristine clinical-scale resin beads. Findings in this work may provide a new strategy for the development of modern resin hemoperfusion technology.