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Detection of a prostate cancer cell line using a bioluminescent affiprobe: An attempt to develop a new molecular probe for ex vivo studies

Author:
Varnosfaderani, Zahra Ghafouri, Emamzadeh, Rahman, Nazari, Mahboobeh, Zarean, Mojtaba
Source:
International journal of biological macromolecules 2019 v.138 pp. 755-763
ISSN:
0141-8130
Subject:
bioluminescence, ex vivo studies, human cell lines, luciferase, monitoring, neoplasm cells, prostatic neoplasms
Abstract:
Molecular probes have become an important tool to decipher cell and molecular events, as well as contributing to clinical diagnosis. In this study, we report on a novel bioluminescence affiprobe and evaluate its functionality by monitoring HER2 expression in the prostate cancer cells. The proposed bioluminescence affiprobe consists of a binding domain, an HER2 specific affibody molecule and a bioluminescent domain, recombinant Renilla luciferase. The experiments indicate that bioluminescence affiprobe can serve as a reliable and user-friendly probe for the detection of human HER2 positive prostate cells as well as ex vivo based detection of HER2-positive human prostate cancer specimens using luminometeric-based assays.
Agid:
6536977