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Trans-3,5-dicaffeoylquinic acid from Geigeria alata Benth. & Hook.f. ex Oliv. & Hiern with beneficial effects on experimental diabetes in animal model of essential hypertension
- Simeonova, Rumyana, Vitcheva, Vessela, Zheleva-Dimitrova, Dimitrina, Balabanova, Vessela, Savov, Ionko, Yagi, Sakina, Dimitrova, Bozhana, Voynikov, Yulian, Gevrenova, Reneta
- Food and chemical toxicology 2019 v.132 pp. 110678
- Geigeria, acarbose, acute oral toxicity, animal disease models, antihypertensive effect, antioxidant enzymes, biomarkers, blood glucose, blood pressure, enzyme inhibition, enzyme inhibitors, glutathione, glutathione peroxidase, glutathione transferase, glutathione-disulfide reductase, glycemic effect, histopathology, hypertension, inhibitory concentration 50, intraperitoneal injection, lethal dose 50, liver, malondialdehyde, mass spectrometry, medicinal plants, noninsulin-dependent diabetes mellitus, nuclear magnetic resonance spectroscopy, oxidative stress, rats, roots, streptozotocin, traditional medicine
- Geigeria alata Benth. & Hook.f. ex Oliv. & Hiern (Asteraceae) is used in Sudanese folk medicine for treatment of diabetes. The study aimed to estimate the acute oral toxicity of trans-3,5-dicaffeoylquinic acid (3,5-diCQA) from G. alata roots and to assess its antihypeglycemic, antioxidant and antihypertensive effects on chemically-induced diabetic spontaneously hypertensive rats (SHRs). The structure of 3,5-diCQA was established by NMR and HRMS spectra. Type 2 diabetes was induced by intraperitoneal injection of streptozotocin. 3,5-diCQA was slightly toxic with LD50 = 2154 mg/kg. At 5 mg/kg 3,5-diCQA reduced significantly (p < 0.05) the blood glucose levels by 42%, decreased the blood pressure by 22% and ameliorated the oxidative stress biomarkers reduced glutathione, malondialdehyde, and serum biochemical parameters. The beneficial effect on antioxidant enzymes was evidenced by the elevated glutathione peroxidase, glutathione reductase, and glutathione S-transferase activitiy in the livers of diabetic animals. 3,5-diCQA prevents the histopathological changes related to diabetes and hypertension. 3,5-diCQA was more potent α-glucosidase inhibitor (IC50 27.24 μg/mL) than acarbose (IC50 99.77 μg/mL). The antihyperglycemic action of the compound was attributed to the α-glucosidase inhibition. The beneficial effects of 3,5-diCQA on streptozotocin-induced diabetic hypertensive rats support the traditional use of G.alata for the management of diabetes.