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Effects of Amanita muscaria extract on different in vitro neurotoxicity models at sub-cellular and cellular levels

Kondeva-Burdina, Magdalena, Voynova, Maria, Shkondrov, Aleksandar, Aluani, Denitsa, Tzankova, Virginia, Krasteva, Ilina
Food and chemical toxicology 2019 v.132 pp. 110687
Amanita muscaria, antioxidant activity, brain, cell lines, free radical scavengers, humans, microsomes, mitochondria, models, mushrooms, neuroprotective effect, neurotoxicity, patients, rats, speech, synaptosomes
Muscimol is the main compound found in Amanita muscaria. Several studies have proven that muscimol has suppressive effects on essential tremor, without impairing speech and coordination. The effects of muscimol in Parkinson-affected patients is also described in a number of studies. These studies describe the free radical scavenging and antioxidant activity of the mushroom extract. We have evaluated the possible neuroprotective effects of a standardized extract from A. muscaria, containing high amounts of muscimol, on different models of neurotoxicity in rat brain microsomes, mitochondria, synaptosomes as well as on neuroblastoma cell line SH-SY5Y. The possible inhibitory effect on human recombinant monoaminoxidase-B (hMAOB) enzyme was also studied. The extract revealed statistically significant neuroprotective effects on the in vitro neurotoxicity models and no inhibitory activity on hMAOB.