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Synthesis and correction of albumin magnetic nanoparticles with organic compounds for absorbing and releasing doxorubicin hydrochloride

Hormozi, Negin, Esmaeili, Akbar
Colloids and surfaces 2019 v.182 pp. 110368
Fourier transform infrared spectroscopy, X-ray diffraction, adverse effects, atomic absorption spectrometry, atomic force microscopy, coprecipitation, cytotoxicity, doxorubicin, human serum albumin, magnetism, magnetite, nanoparticles, neoplasm cells, pH, response surface methodology, scanning electron microscopy, system optimization, temperature, toxicity testing, ultraviolet-visible spectroscopy
In an ideal delivery system, carrier nanoparticles are used as a promising alternative with minimized adverse effects to treat a variety of diseases. The purpose of this study was to create a targeted delivery system for doxorubicin hydrochloride (DOX−HCl), using Fe3O4-L@HSA-β-cyclodextrin (β-CD)/Allyl amine nanoparticles. In this study, magnetite nanoparticles (Fe3O4) were produced by co-precipitation, while albumin nanoparticles (HSA) were produced by the desolvation method. The properties of the nanoparticles were studied by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and vibrating sample magnetometer (VSM). To evaluate the loading of the drug on the synthesized nanoparticles, ultraviolet-visible spectrophotometry (UV–Vis) and atomic force microscopy (AFM) were used. DOX−HCl loading was studied by two experiments: an optimization method (OM) and a one-factor-at-a-time method (OFATM). Response surface methodology (RSM) was utilized to optimize the parameters. The optimal conditions for drug loading of nanoparticles in OM and OFATM methods were 81.46% and 77%, respectively. The release of DOX−HCl drug from the synthesized nanoparticles at a temperature of 37 °C and specific time in pH 5.3 and pH 7.4 was 83.35% and 38.39%, respectively. To examine the cytotoxicity of nanoparticles with drugs, the MTT assay was performed using MCF-7 cancer cells. Finally, cell uptake was tested using inductively coupled plasma-mass spectrometry (ICP-MS).