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Ethanol production from water hyacinth (Eichhornia crassipes) hydrolysate by hyper-thermal acid hydrolysis, enzymatic saccharification and yeasts adapted to high concentration of xylose
- Sunwoo, InYung, Kwon, Jeong Eun, Nguyen, Trung Hau, Jeong, Gwi-Tack, Kim, Sung-Koo
- Bioprocess and biosystems engineering 2019 v.42 no.8 pp. 1367-1374
- Clavispora lusitaniae, Eichhornia crassipes, Saccharomyces cerevisiae, Scheffersomyces stipitis, acid hydrolysis, ethanol, ethanol production, evolutionary adaptation, feedstocks, fermentation, hydrolysates, saccharification, slurries, sulfuric acid, xylose, yeasts
- Water hyacinth (Eichhornia crassipes) was used as a feedstock for ethanol production. The optimal hyper-thermal (HT) acid hydrolysis conditions were 8% (w/v) slurry content, 200 mM H₂SO₄, at 160 °C for 20 min and enzymatic saccharification for 48 h using an enzyme mixture of 20 units/mL Viscozyme L and Cellic C Tec2. After pretreatment, 48.2 g/L monosaccharides were obtained. Fermentation was conducted with wild and adapted Saccharomyces cerevisiae, Pichia stipitis and Candida lusitaniae. Wild-type S. cerevisiae, P. stipitis, and C. lusitaniae produced 15.3, 19.5 and 22.7 g/L of ethanol, respectively. Adaptive evolution was carried out on 6% (w/v) xylose. S. cerevisiae, P. sipitis and C. lusitaniae adapted to xylose produced 15.3, 21.4 and 23.9 g/L of ethanol with YEₜOH of 0.32, 0.44 and 0.49, respectively. These results indicate that water hyacinth has potential as a feed stock for ethanol.