Main content area

Efficient production of coenzyme Q₁₀ from acid hydrolysate of sweet sorghum juice by Rhodobacter sphaeroides

Wang, Y., Chen, S., Liu, J., Lv, P., Cai, D., Zhao, G.
RSC advances 2019 v.9 no.39 pp. 22336-22342
Rhodobacter sphaeroides, batch fermentation, biorefining, biotransformation, corn, hydrolysates, juices, nitrogen, pH, sugars, sweet sorghum, temperature
In order to achieve efficient bioconversion of biomass-derived sugars, acid hydrolysate of sweet sorghum juice (SSJAH) containing abundant fermentable sugars was used for coenzyme Q₁₀ (CoQ₁₀) fermentation by Rhodobacter sphaeroides CQ-09-1. The synthesis of CoQ₁₀ was facilitated when the initial concentration of total sugar was 80.00 g L⁻¹. And the highest CoQ₁₀ titer was obtained when the pH and temperature were maintained at 7.00 and 30.00 °C, respectively. Moreover, corn steep powder (CSP) was proved to be an efficient nitrogen & salt supplement to SSJAH. Under the optimized conditions, the titer of CoQ₁₀ reached 141.95 mg L⁻¹ in a fed-batch fermentation. The CoQ₁₀ titer reported was about two times higher than that obtained in the previous study using wild strains. This process introduces a potential way to produce CoQ₁₀ using the concept of biorefinery, while making full use of sweet sorghum juice (SSJ).