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Influence of Lactobacillus plantarum individually and in combination with low O2-MAP on the pathogenic potential of Listeria monocytogenes in cabbage

Dong, Qingli, Zhang, Wenmin, Guo, Liang, Niu, Hongmei, Liu, Qing, Wang, Xiang
Food control 2020 v.107 pp. 106765
Lactobacillus plantarum, Listeria monocytogenes, cabbage, carbon dioxide, gastrointestinal system, gene expression regulation, listeriosis, oxygen, quantitative polymerase chain reaction, resistance genes, reverse transcriptase polymerase chain reaction, reverse transcription, storage temperature, storage time, stress tolerance, virulence
To verify the effect of Lactobacillus plantarum subsp. plantarum CICC 6257 individually (T₁) and in combination with low O₂-MAP (3% O₂/10% CO₂/87% N₂) (T₂) on the pathogenic potential of Listeria monocytogenes in cabbage stored at 10 °C, the potential for growth, the ability to survive a simulated gastrointestinal tract and the expression of virulence genes of L. monocytogenes were analyzed during 6 days of storage. It indicated that the growth of L. monocytogenes was significantly (p < 0.05) inhibited by T₁, with more pronounced inhibition effect was achieved by T₂, at the end of storage. Moreover, both T₁ and T₂ treatment could significantly (p < 0.05) decrease the survival ratio of L. monocytogenes during passage through the simulated gastrointestinal tract. Meanwhile, reverse transcription quantitative real-time PCR (RT-qPCR) revealed that the stress resistance gene (sigB) was up-expressed (p < 0.05) in T₂ than that in T₁ at the end of storage. Moreover, neither T₁ nor T₂ had inhibition effect on the relative expression of hly gene. The other three virulence genes, inlA, inlB and prfA, were relatively down-expressed in T₂ than that in T₁ on day 1, while relative up-regulation in T₂ compared with that in T₁ was observed on day 6. These results indicated that application of L. plantarum to cabbage could be a potential bio-approach to decrease the pathogenic ability of L. monocytogenes, which is important for reducing the development of listeriosis. In addition, it is noteworthy that O₂ availability conditions could influence the preservative efficacy on L. monocytogenes.