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Influence of Lactobacillus plantarum individually and in combination with low O2-MAP on the pathogenic potential of Listeria monocytogenes in cabbage

Author:
Dong, Qingli, Zhang, Wenmin, Guo, Liang, Niu, Hongmei, Liu, Qing, Wang, Xiang
Source:
Food control 2020 v.107 pp. 106765
ISSN:
0956-7135
Subject:
Lactobacillus plantarum, Listeria monocytogenes, cabbage, carbon dioxide, gastrointestinal system, gene expression regulation, listeriosis, oxygen, quantitative polymerase chain reaction, resistance genes, reverse transcriptase polymerase chain reaction, reverse transcription, storage temperature, storage time, stress tolerance, virulence
Abstract:
To verify the effect of Lactobacillus plantarum subsp. plantarum CICC 6257 individually (T1) and in combination with low O2-MAP (3% O2/10% CO2/87% N2) (T2) on the pathogenic potential of Listeria monocytogenes in cabbage stored at 10 °C, the potential for growth, the ability to survive a simulated gastrointestinal tract and the expression of virulence genes of L. monocytogenes were analyzed during 6 days of storage. It indicated that the growth of L. monocytogenes was significantly (p < 0.05) inhibited by T1, with more pronounced inhibition effect was achieved by T2, at the end of storage. Moreover, both T1 and T2 treatment could significantly (p < 0.05) decrease the survival ratio of L. monocytogenes during passage through the simulated gastrointestinal tract. Meanwhile, reverse transcription quantitative real-time PCR (RT-qPCR) revealed that the stress resistance gene (sigB) was up-expressed (p < 0.05) in T2 than that in T1 at the end of storage. Moreover, neither T1 nor T2 had inhibition effect on the relative expression of hly gene. The other three virulence genes, inlA, inlB and prfA, were relatively down-expressed in T2 than that in T1 on day 1, while relative up-regulation in T2 compared with that in T1 was observed on day 6. These results indicated that application of L. plantarum to cabbage could be a potential bio-approach to decrease the pathogenic ability of L. monocytogenes, which is important for reducing the development of listeriosis. In addition, it is noteworthy that O2 availability conditions could influence the preservative efficacy on L. monocytogenes.
Agid:
6544832