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Volatiles and functional peptides compositions of Trichoderma variants induced by a new strategy of irradiation

Author:
Abd Alla El-Bialy, Heba, Adel-Fattah Mohamed Shahin, Azza, El-Fouly, Mohie Zohier, Awad, Mohamed Ahmed, Khalifa, EL-Said Zaki, Fahmy, Shimaa Mohamed
Source:
Biocatalysis and agricultural biotechnology 2019 v.20 pp. 101261
ISSN:
1878-8181
Subject:
Alternaria solani, Fusarium oxysporum, Solanum lycopersicum var. lycopersicum, Thanatephorus cucumeris, Trichoderma koningii, Trichoderma viride, amino acids, antibiosis, antifungal properties, biological control, fungal antagonists, fungi, gamma radiation, gas chromatography-mass spectrometry, irradiation, metabolites, mutation, pathogens, peptides, seeds, soil, tomatoes, volatile compounds
Abstract:
Induction of mutation using gamma radiation improves the efficiency of Trichoderma against tomato fungal pathogens (Alternaria solani, Fusarium oxysporium, and Rhizoctonia solani). Results of the present study reveal the antagonistic activity of selected 2nd generation variants is higher than their 1st generation variants and the activity of latter variants is higher than their parent strains. The metabolites derived from irradiated variants are identified to understand their role in antibiosis interactions. GC/MS profile of volatiles emitted from T. viride Td₁ and as its variants shows they are different in composition. The present study clears the potency of volatiles emitted from 2nd generation variant; T. viride Td₁/2–3 to control tomato fungal pathogens using two strategies; applying the volatile compounds to tomato seeds or to soil infested with fungal pathogens. Results also showed the superiority of functional peptides originates from an irradiated variant; T. koningii Tk₁/3-3 to control tomato fungal pathogens especially peptide fraction; F₄ that characterized using LC/MS to fifteen sub-fractions having 12 amino acid residues or less; one of them is identified as peptabiotic.
Agid:
6545289