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Redox status and fatty acid composition of Mactra corallina digestive gland following exposure to acrylamide

Author:
Trabelsi, Wafa, Chetoui, Imene, Fouzai, Chaima, Bejaoui, Safa, Rabeh, Imen, Telahigue, Khaoula, Chalghaf, Mohamed, El Cafsi, Mhamed, Soudani, Nejla
Source:
Environmental science and pollution research international 2019 v.26 no.22 pp. 22197-22208
ISSN:
0944-1344
Subject:
Mactra, acetylcholinesterase, acrylamides, arachidonic acid, ascorbic acid, catalase, clams, cytotoxicity, docosahexaenoic acid, eicosapentaenoic acid, enzyme activity, fatty acid composition, glutathione, glutathione peroxidase, hydrogen peroxide, industrial applications, linoleic acid, malondialdehyde, metallothionein, oxidative stress, superoxide dismutase
Abstract:
Acrylamide (ACR), a ubiquitous agent, has various chemical and industrial applications, and it is found in backed or fried carbohydrate-rich food. It has been related to multiple toxicological effects, and it causes high cytotoxicity through oxidative stress. The present study aimed to investigate the potential effect of ACR toxicity administered at different concentrations (5, 10, and 20 mg/L), during 5 days, in order to evaluate the fatty acid (FA) composition and redox state in the digestive gland of Mactra corallina. The results showed, in ACR-treated clams, a significant increase in malondialdehyde, hydrogen peroxide, protein carbonyl, and metallothionein levels, as well as an alteration of the enzymatic (superoxide dismutase, glutathione peroxidase, and catalase) and non-enzymatic (reduced glutathione and ascorbic acid) antioxidant status. However, acetylcholinesterase activity was inhibited in a concentration-dependent manner. In our experiment, the n-3 (Omega-3) and n-6 (Omega-6) polyunsaturated fatty acid levels were significantly changed in all ACR-treated groups. A decrease in eicosapentaenoic acid (C20:5n-3, EPA) and docosahexaenoic acid (C22:6n-3, DHA) was observed in 10-mg/L and 20-mg/L ACR-treated groups. Nevertheless, arachidonic acid (C20:4n-6, ARA) and its precursor linoleic acid (C18:2n-6, LA) were increased. Besides oxidative stress parameters, FA composition may be an additional tool for assessing ACR contamination.
Agid:
6548498