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Dissection of loci conferring resistance to stripe rust in Chinese wheat landraces from the middle and lower reaches of the Yangtze River via genome-wide association study

Author:
Cheng, Yukun, Li, Jian, Yao, Fangjie, Long, Li, Wang, Yuqi, Wu, Yu, Li, Jing, Ye, Xueling, Wang, Jirui, Jiang, Qiantao, Kang, Houyang, Li, Wei, Qi, Pengfei, Liu, Yaxi, Deng, Mei, Ma, Jian, Jiang, Yunfeng, Chen, Xianming, Zheng, Youliang, Wei, Yuming, Chen, Guoyue
Source:
Plant science 2019 v.287 pp. 110204
ISSN:
0168-9452
Subject:
Puccinia striiformis f. tritici, Triticum, cultivars, data collection, disease resistance, field experimentation, foliar diseases, fungi, genome-wide association study, genomics, haplotypes, landraces, loci, mature plants, microsatellite repeats, pathogens, phenotype, phenotypic variation, quantitative trait loci, races, resistance genes, stripe rust, wheat, China
Abstract:
Stripe rust (Yr), caused by the fungal pathogen Puccinia striiformis f. sp. tritici, is a devastating foliar disease of wheat in China. Chinese wheat landraces originating from the middle and lower reaches of the Yangtze River are potential stripe-rust resistance resources. To identify APR genes for stripe-rust resistance, a panel of 188 accessions derived from the middle and lower reaches of the Yangtze River were inoculated with a mixture of Chinese P. striiformis f. sp. tritici races and resistance evaluated under field conditions in five environments at adult-plant stages. Seventy-three accessions showed degrees of stable resistance. Combining phenotypic datasets from multiple field experiments with high-quality Diversity Arrays Technology and simple sequence repeat markers, we detected 21 marker-trait associations spanning 18 quantitative trait loci (QTLs) on chromosomes 1B, 2A, 2B, 3B, 4A, 5A, 5B, 6B, and 6D, respectively. Single QTLs explained 9.67% to 16.14% of the observed phenotypic variation. Nine QTLs co-localized with previously reported Yr genes or genomic regions. The remaining QTLs were potential novel loci associated with adult-stage stripe-rust resistance. Two novel QTLs, QYr.sicau-3B.2 and QYr.sicau-5B.3, located on chromosomes 3B and 5B significantly explained 16.14% and 11.16% of the phenotypic variation, respectively. Haplotype analysis revealed that accessions carrying APR variants or their combinations showed enhanced degrees of resistance. The potentially novel loci or genomic regions associated with adult-stage resistance may be useful to improve stripe-rust resistance in current wheat cultivars and for future isolation of stripe-rust resistance genes.
Agid:
6552999