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The potential use of methyl jasmonate, putrescine and cold atmospheric plasma on genetic variability and seedling growth improvement in medicinal plant Catharanthus roseus L. cultivar

Mohammadzadeh-Shahir, Mahnoosh, Noormohammadi, Zahra, Farahani, Farah, Atyabi, Seyed Mohammad
Industrial crops and products 2019 v.140 pp. 111601
Catharanthus roseus, catalase, cold, enzyme activity, genetic markers, genetic polymorphism, genetic variation, indole alkaloids, leaf length, medicinal plants, methyl jasmonate, monoterpenoids, peroxidase, putrescine, seed germination, seedling growth
Madagascar Periwinkle (Catharanthus roseus) as a medicinal plant produces more than 100 monoterpenoid indole alkaloids. In present study, six groups including; control, Cold atmospheric plasma, methyl jasmonate hormone, putrecsine, combination of methyl jasmonate and cold plasma, and putrecsine and cold plasma treated plants were studied. Seed germination, morphological traits, catalase and peroxidase enzyme activities and amount of total alkaline were investigated in treated plants. Genetic variation of treated and control plants were evaluated by 21 coding targeted markers; Sequence related amplified polymorphism (SRAP) and Target related amplified polymorphism (TRAP). Cold plasma + putrescine treated plants showed highest amount of stem length as well as leaf length and wide size. Peroxidase and catalase activities were significantly increased in treated plants of putrescine + cold plasma and methyl jasmonate treated plants respectively. Total alkaline was significantly higher in treated plants of putrescine, and putrescine + cold plasma. In both TRAP and SRAP molecular markers, treatments of methyl jasmonate + plasma and putrescine + cold plasma had the highest level of genetic polymorphism and both markers showed moderate discriminating power to differentiate plants treated from each other. The putrescine + cold plasma is one of the best treatments for improved germination rate and seedling growth as well as enzyme activity and amount of alkaloid in C. roseus plants as well as higher genetic variation.