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Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery
- Jimenez-Falcao, Sandra, Joga, Natalia, García-Fernández, Alba, Llopis Lorente, Antoni, Torres, Daniel, de Luis, Beatriz, Sancenón, Félix, Martínez-Ruiz, Paloma, Martínez-Máñez, Ramón, Villalonga, Reynaldo
- Journal of materials chemistry B 2019 v.7 no.30 pp. 4669-4676
- benzimidazole, beta-fructofuranosidase, doxorubicin, gluconic acid, glucose, glucose oxidase, gold, hydrolysis, nanocarriers, nanogold, nanoparticles, neoplasm cells, neoplasms, porous media, silica, sucrose
- We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au–mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells.