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Characterization and complete genome sequence analysis of phage GP4, a novel lytic Bcep22-like podovirus

Author:
Wang, Ronghuan, Cong, Yu, Mi, Zhiqiang, Fan, Hang, Shi, Taoxing, Liu, Hui, Tong, Yigang
Source:
Archives of virology 2019 v.164 no.9 pp. 2339-2343
ISSN:
0304-8608
Subject:
DNA, Podoviridae, Ralstonia solanacearum, bacteriophages, cysteine, genetic databases, genome, high-throughput nucleotide sequencing, latent period, open reading frames, phylogeny, phylotype, temperature, transfer RNA
Abstract:
We isolated a novel lytic phage of Ralstonia solanacearum, GP4. The GP4 phage has a latent period of ~ 2 h at its optimal multiplicity of infection and is stable at temperatures ranging from 40–70 °C. GP4 lysed 16 strains of R. solanacearum belonging to phylotype IV. High-throughput sequencing revealed that GP4 has a linear dsDNA genome that consists of 61,129 bp, contains 83 open reading frames, and encodes a tRNA for cysteine. The GP4 genome has low similarity to other phage sequences in the GenBank database. Phylogenetic analysis indicated that GP4 can be taxonomically classified as a member of the Bcep22-like subfamily of the family Podoviridae.
Agid:
6557057