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Dendritic core-shell rhodium@platinum-cobalt nanocrystals for ultrasensitive electrochemical immunoassay of squamous cell carcinoma antigen
- Chen, Yao, Feng, Jiu-Ju, Mei, Li-Ping, Shi, Chuan-Guo, Wang, Ai-Jun
- Journal of colloid and interface science 2019 v.555 pp. 647-654
- antigens, biomarkers, blood serum, catalytic activity, catechol, detection limit, disease surveillance, electrochemistry, immunoassays, immunosensors, nanocrystals, oxidation, rhodium, squamous cell carcinoma
- Squamous cell carcinoma antigen (SCCA) has great diagnostic values for its high specificity (90–96%) in diagnosis of squamous cell carcinoma especially cancers, where electrochemical immunoassay is powerful for its accurate and reliable detection of specific tumor biomarkers at very low levels. In this work, well-defined three-dimensional dendritic core-shell rhodium@platinum-cobalt nanocrystals (Rh@PtCo NCs) were facilely prepared via a simple one-pot solvothermal strategy. Taking advantage of dramatically enhanced catalytic activity of the Rh@PtCo NCs for catechol oxidation, a label-free electrochemical immunosensor was developed for highly sensitive assay of SCCA. Under optimal conditions, the electrochemical signals were linearly correlated with the logarithm of the SCCA concentration, showing a broad linear range from 0.0001 to 10.0 ng mL−1 and a ultralow detection limit of 0.04 pg mL−1 (S/N = 3). The resultant immunosensor was further exploited for actual analysis of serum samples with convinced results. This immunosensor has good expectation in actual samples analysis and provides a universal approach for detection of other tumor markers and disease surveillance.