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Isolation of Bisphenol A-Tolerating/degrading Shewanella haliotis Strain MH137742 from an Estuarine Environment

de Santana, Felipe Silva, Gracioso, Louise Hase, Karolski, Bruno, dos Passos Galluzzi Baltazar, Marcela, Mendes, Maria Anita, do Nascimento, Claudio Augusto Oller, Perpetuo, Elen Aquino
Applied biochemistry and biotechnology 2019 v.189 no.1 pp. 103-115
Haliotis, Shewanella, bacteria, biochemical pathways, bioremediation, bisphenol A, carbon, culture media, endocrine system, endocrine-disrupting chemicals, environmental degradation, estuaries, liquid chromatography, liquids, matrix-assisted laser desorption-ionization mass spectrometry, metabolites, plasticizers, ribosomal RNA, salt tolerance, sequence analysis
The human exposure to bisphenol A (BPA) occurs frequently. Once, this compound was one of the highest volume chemicals produced worldwide and used as a plasticizer in many products. However, even at low concentration, it can cause severe damage to the endocrine system because of its endocrine disruptor activity. Thus, mitigation studies to remove or reduce this contaminant from the environment are essential. An alternative method of removing it from the environment is the use of bioremediation processes to the selected bacteria isolated from a BPA-impacted area. In this work, four halotolerant strains were isolated from the Santos Estuary System, one of the most important Brazilian examples of environmental degradation. In the present work, one strain presented strong BPA tolerance and high BPA-degrading activity and could grow in a minimum medium containing BPA as the main carbon source. Strain MH137742 was identified as Shewanella haliotis, based on 16S rRNA gene sequencing and mass spectrometry identification by MALDI-TOF Biotyper. Shewanella haliotis was able to tolerate up to 150 mg L⁻¹ of BPA and biotransform 75 mg L⁻¹ in 10 h in a liquid culture medium. Based on the analysis of the produced metabolites by LC-MS, it was possible to predict the metabolic pathway used by this microorganism to degrade the BPA.