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Assay design considerations for use of affinity aptamer amplification in ultra-sensitive protein assays using capillary electrophoresis
- Janssen, Kris P. F., Knez, Karel, Pollet, Jeroen, Roberts, Scott J., Schrooten, Jan, Lammertyn, Jeroen
- Analytical methods 2011 v.3 no.9 pp. 2156-2159
- capillary electrophoresis, gene amplification, oligonucleotides, proteins
- Affinity probe capillary electrophoresis (APCE) holds promise to be a powerful tool in diagnostic applications that rely on the selective and sensitive detection of proteins. Aptamers can serve to unlock the full potential of the technology since their use opens up possibilities to unite the immuno-sensing and separating strength of capillary electrophoresis with the signal enhancement power of nucleic acid amplification. To enable routine use of aptamer APCE, careful selection of the experimental conditions is crucial. Here it is shown how customization and careful consideration of the experimental parameters can significantly improve assay performance.