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Citrate-based Fluorophore-Modified Cellulose Nanocrystals as A Biocompatible Fluorescent Probe for Detecting Ferric Ions and Intracellular Imaging

Author:
Chen, Heng, Huang, Junxian, Hao, Beibei, Yang, Boguang, Chen, Shaojun, Yang, Guanghui, Xu, Jianbin
Source:
Carbohydrate polymers 2019
ISSN:
0144-8617
Subject:
acid hydrolysis, cellulose, citric acid, cytotoxicity, fluorescence, fluorescent dyes, image analysis, ions, iron, nanocrystals, photostability, sulfuric acid, wavelengths
Abstract:
In this contribution, citrate-based fluorophore (CF)-modified cellulose nanocrystals (CNCs) were prepared in a facile manner using sulfuric acid hydrolysis of citric acid/cysteine-treated microcrystalline celluloses. These rod-like CNCs have an average length of 156 nm and an average width of 7.9 nm. Because of conjugated CFs, these CNCs exhibit typical fluorescence characteristics, including a maximum excitation wavelength at 350 nm, maximum emission wavelength at 435 nm, high quantum yield of 83 %, and good photostability. More importantly, these fluorescent CNCs exhibit a selective quenching effect toward Fe3+ ions; meanwhile, these CNCs exhibit negligible cytotoxicity and were internalized by cells. Therefore, these CNCs can be used as a fluorescence probe for detecting Fe3+ ions in living cells.
Agid:
6569908