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Quantitative detection of severe fever with thrombocytopenia syndrome virus via electrochemiluminescence immunoassay
- Liang, Xiu-Li, Liu, Jin-Xia, Jiao, Yong-Jun, Zhu, Jin, Ding, Shou-Nian
- Analytical methods 2019 v.11 no.32 pp. 4197-4203
- analytical methods, blood serum, detection limit, electrochemiluminescence, fever, immunoassays, immunosensors, iron oxides, magnetism, quantitative analysis, quantum dots, silica, thrombocytopenia, viruses
- In this work, a sensitive method based on the electrochemiluminescence (ECL) technique was proposed to quantitatively detect severe fever with thrombocytopenia syndrome virus (SFTSV) for the first time. A sandwich-type immunosensor was prepared by utilizing Fe₃O₄@SiO₂ as a magnetic sensing platform and CdZnTeS quantum dots (QDs) as labels. Under the optimal experimental conditions, the fabricated ECL immunosensor showed excellent performance for SFTSV detection. The limit of detection (LOD) was 0.003 fg mL⁻¹ (S/N = 3), so the strategy presented in this study is the first quantitative analysis of SFTSV with a lower LOD. In addition, the developed SFTSV immunosensor exhibited long-term stability, high reproducibility and great specificity. Furthermore, serum sample analysis was performed to evaluate the practical application of the fabricated immunosensor. The obtained results manifested that the proposed ECL immunosensor may have great application prospects in clinical diagnosis.