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Highly selective and wash-free visualization of resistant bacteria with a relebactam-derived fluorogenic probe

Author:
Chen, Yefeng, Xu, Minqiu, Xu, Weipan, Song, Heng, Hu, Liqiang, Xue, Shuyuan, Zhang, Shuangzhan, Qian, Xiana, Xie, Hexin
Source:
Chemical communications 2019 v.55 no.67 pp. 9919-9922
ISSN:
1364-548X
Subject:
antibiotic resistance, bacteria, beta-lactam antibiotics, beta-lactamase, chemical bonding, chemical compounds, chemical reactions, fluorescence, fluorescent dyes, image analysis, serine
Abstract:
Reported herein is a relebactam-derived fluorogenic reagent for covalent labeling of serine β-lactamases (SBLs), which are the major causes of bacterial resistance to β-lactam antibiotics. This highly selective imaging reagent generates over 300-fold stronger near-infrared fluorescence signals upon covalently bonding to SBLs, allowing wash-free visualization of live antimicrobial-resistant bacteria.
Agid:
6579256