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Dual RNA-seq uncovers the immune response of Larimichthys crocea to the secY gene of Pseudomonas plecoglossicida from the perspective of host-pathogen interactions

Wang, Luying, Sun, Yunjia, Zhao, Lingmin, Xu, Xiaojin, Huang, Lixing, Qin, Yingxue, Su, Yongquan, Zhang, Jiaonan, Yan, Qingpi
Fish & shellfish immunology 2019 v.93 pp. 949-957
Larimichthys crocea, Pseudomonas plecoglossicida, RNA interference, Toll-like receptors, aerobes, bacteria, bacterial infections, bacterial proteins, coagulation, complement, fish diseases, genes, host-pathogen relationships, immune response, marine fish, messenger RNA, mortality, pathogens, protein secretion, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, sequence analysis, signal transduction, spleen, transcriptome, virulence
Pseudomonas plecoglossicida is a Gram-negative aerobic bacterium that causes high mortality and serious economic losses in some commercial marine fish. Expression of secY was found to be significantly upregulated at 18 °C compared to 28 °C by RNA-seq and qRT-PCR. All five tested recombinant vectors (pCM130/tac + shRNA) significantly reduced secY mRNA levels in P. plecoglossicida. The recombinant vector encoding shRNA-1165 exhibited the best gene-silencing efficiency, 82.4% and was used to create an RNAi strain for further studies. Compared with the wildtype strain, infections of Larimichthys crocea with the RNAi strain resulted in a 2-day delay in onset time and a 35% reduction in mortality, as well as the alleviation of spleen symptoms. The spleens of L. crocea infected by the wild type or RNAi strain of P. plecoglossicida were subjected to dual RNA-seq at 2 dpi. Compared with the wildtype strain, infection of P. plecoglossicida with the RNAi strain resulted in significant changes in the transcriptomes of both host and pathogen. KEGG analysis showed that the complement and coagulation cascade and the Toll-like receptor signalling pathway were the most enriched host pathways. In the pathogen, genes of the “Sec secretion system” were significantly downregulated. This downregulation of “Sec secretion system” genes hindered the secretion of bacterial proteins and reduced the virulence of P. plecoglossicida. Thus, it was easier for L. crocea to clear the RNAi strain of P. plecoglossicida, and the immune response was similarly reduced. The results indicated that secY was a virulence gene of P. plecoglossicida and played roles in the host-pathogen interactions of L. crocea and P. plecoglossicida.