Reconstitution of the pyridoxal 5′-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis
- Source:
- Chemical communications 2013 v.49 no.63 pp. 7058-7060
- ISSN:
- 1364-548X
- Subject:
- biosynthesis, catalytic activity, chemical communication, chemical reactions, moieties, phosphates, pyridoxal, pyridoxal phosphate, pyridoxine, serine, serine C-palmitoyltransferase, sphingolipids, substrate specificity
- Abstract:
- The pyridoxal 5′-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT) is required for de novo sphingolipid biosynthesis. A previous study revealed a novel and unexpected interaction between the hydroxyl group of the l-serine substrate and the 5′-phosphate group of PLP. By using pyridoxal (PL), the dephosphorylated analogue of vitamin B₆, we show here that this interaction is important for substrate specificity and optimal catalytic efficiency.
- Agid:
- 6598837
- https://doi.org/10.1039/c3cc43001d