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The determination of DNA methyltransferase activity by quenching of tris(2,2′-bipyridine)ruthenium electrogenerated chemiluminescence with ferrocene

Author:
Luo, Xiaoe, Li, Yan, Zheng, Jianbin, Qi, Honglan, Liang, Zhenxing, Ning, Xiaohui
Source:
Chemical communications 2015 v.51 no.46 pp. 9487-9490
ISSN:
1364-548X
Subject:
DNA, adenine, detection limit, electrochemiluminescence, enzyme activity, ferrocenes, methylation, methyltransferases, nanogold, pyridines, ruthenium
Abstract:
An electrogenerated chemiluminescence (ECL) biosensing method for the determination of DNA methyltransferase activity is developed by the quenching of tris(2,2′-bipyridine)ruthenium ECL by ferrocene, and it is demonstrated that the ECL biosensing method measures DNA adenine methylation methyltransferase over a dynamic concentration range (0.1 U mL⁻¹–100 U mL⁻¹) with an extremely low detection limit of 0.03 U mL⁻¹, using gold nanoparticles and a quenching ECL signal produced by a chemical quencher such as ferrocene.
Agid:
6609762