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Coumarin-based fluorescent ‘AND’ logic gate probes for the detection of homocysteine and a chosen biological analyte

Author:
Wu, Luling, Gardiner, Jordan E., Kumawat, Lokesh K., Han, Hai-Hao, Guo, Ruiying, Li, Xin, He, Xiao-Peng, Elmes, Robert B. P., Sedgwick, Adam C., Bull, Steven D., James, Tony D.
Source:
RSC advances 2019 v.9 no.45 pp. 26425-26428
ISSN:
2046-2069
Subject:
fluorescence, fluorescent dyes, homocysteine, nitroreductases
Abstract:
With this research we set out to develop a number of coumarin-based ‘AND’ logic fluorescence probes that were capable of detecting a chosen analyte in the presence of HCys. Probe JEG-CAB was constructed by attaching the ONOO⁻ reactive unit, benzyl boronate ester, to a HCys/Cys reactive fluorescent probe, CAH. Similarly, the core unit CAH was functionalised with the nitroreductase (NTR) reactive p-nitrobenzyl unit to produce probe JEG-CAN. Both, JEG-CAB and JEG-CAN exhibited a significant fluorescence increase when exposed to either HCys and ONOO⁻ (JEG-CAB) or HCys and NTR (JEG-CAN) thus demonstrating their effectiveness to function as AND logic gates for HCys and a chosen analyte.
Agid:
6611957