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Intracellular localization of histone deacetylase HDA6 in plants

Author:
Kurita, Kazuki, Sakamoto, Yuki, Naruse, Sota, Matsunaga, Tomoko M., Arata, Hideyuki, Higashiyama, Tetsuya, Habu, Yoshiki, Utsumi, Yoshinori, Utsumi, Chikako, Tanaka, Maho, Takahashi, Satoshi, Kim, Jong-Myong, Seki, Motoaki, Sakamoto, Takuya, Matsunaga, Sachihiro
Source:
Journal of plant research 2019 v.132 no.5 pp. 629-640
ISSN:
0918-9440
Subject:
Arabidopsis thaliana, acetates, acetic acid, acetylation, acid treatment, cassava, chromocenters, drought tolerance, epigenetics, eukaryotic cells, gene silencing, genes, heterochromatin, histone acetyltransferase, histone code, histone deacetylase, histones, image analysis, models, rice, root meristems, water stress
Abstract:
Histone modification is an important epigenetic mechanism in eukaryotes. Histone acetyltransferase and deacetylase regulate histone acetylation levels antagonistically, leading to dynamic control of chromatin structure. One of the histone deacetylases, HDA6, is involved in gene silencing in the heterochromatin regions, chromocenter formation, and metabolic adaptation under drought stress. Although HDA6 plays an important role in chromatin control and response to drought stress, its intracellular localization has not been observed in detail. In this paper, we generated transformants expressing HDA6-GFP in the model plant, Arabidopsis thaliana, and the crops, rice, and cassava. We observed the localization of the fusion protein and showed that HDA6-GFP was expressed in the whole root and localized at the nucleus in Arabidopsis, rice, and cassava. Remarkably, HDA6-GFP clearly formed speckles that were actively colocalized with chromocenters in Arabidopsis root meristem. In contrast, such speckles were unlikely to be formed in rice or cassava. Because AtHDA6 directly binds to the acetate synthesis genes, which function in drought tolerance, we performed live imaging analyses to examine the cellular dynamics of pH in roots and the subnuclear dynamics of AtHDA6 responding to acetic acid treatment. The number of HDA6 speckles increased during drought stress, suggesting a role in contributing to drought stress tolerance.
Agid:
6617909