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Comparative Evaluation on Vitamin E and Carotenoid Accumulation in Sweet Corn (Zea mays L.) Seedlings under Temperature Stress

Xiang, Nan, Li, Chunyan, Li, Gaoke, Yu, Yongtao, Hu, Jianguang, Guo, Xinbo
Journal of agricultural and food chemistry 2019 v.67 no.35 pp. 9772-9781
Zea mays, biosynthesis, carotenoids, gene expression, high performance liquid chromatography, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, seedlings, sweetcorn, temperature, transcription (genetics), vitamin E
This study aims to investigate the response profiles of vitamin E and carotenoids on transcription and metabolic levels of sweet corn seedlings under temperature stress. The treated temperatures were set as 10 °C (low temperature, LT), 25 °C (control, CK), and 40 °C (high temperature, HT) for sweet corn seedlings. The gene expression profiles of vitamin E and carotenoids biosynthesis pathways were analyzed by real time quantitative polymerase chain reaction (RT-qPCR), and the composition profiles were analyzed by high performance liquid chromatography (HPLC). Results showed that vitamin E gradually accumulated in response to LT stress but was limited by HT stress. The increase of carotenoids was suppressed by LT stress whereas HT stress promoted it. The existing results elaborated the interactive and competitive relationships of vitamin E and carotenoids in sweet corn seedlings to respond to extreme temperature stress at transcriptional and metabolic levels. The present study would improve sweet corn temperature resilience with integrative knowledge in the future.