U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Exploring electrochemistry of carbon nanodots and its application in noninvasive bacterial growth monitoring

Ritu Das, Neetu Singh
Biosensors & bioelectronics 2019 v.144 pp. 111640
alginates, antibiotic resistance, antibiotics, bacteria, bacterial growth, biochemical pathways, biosensors, carbon, cost effectiveness, disease progression, electrochemistry, encapsulation, growth models, microparticles, monitoring, nanotechnology, pH, plate count, redox potential, screening
Cost effective and miniaturized methods aiming for high throughput monitoring of bacterial growth are of great significance, especially for tracking disease progression in early stage as well as in screening antibiotic resistant species. Here, we demonstrate an electrochemical platform for noninvasive monitoring of bacterial growth by encapsulating bacterial cells and carbon nanodots in alginate microspheres. The synthesized carbon nanodots have been explored for electrochemical properties, and its redox properties have been utilized for developing bacterial growth monitoring platform. These synthesized CDs are sensitive to pH change and respond as change in redox potential over time as pH of the medium changes due to growth and metabolic activities of bacteria. We determined the bacterial growth kinetics by measuring the redox potential changes of the carbon nanodots over time. The developed platform has been demonstrated to detect the presence of bacteria, the difference in growth rates of bacteria and its susceptibility to the antibiotic with low bacterial counts (10³ CFU) in 20 min; thus, redox properties of CDs has the potential to provide a sensitive detection platform.