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Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response

Sassaki, G.L., Czelusniak, P.A., Vicente, V.A., Zanata, S.M., Souza, L.M., Gorin, P.A.J., Iacomini, M.
International journal of biological macromolecules 2011 v.48 no.1 pp. 177-182
Exophiala, animal pathogenic fungi, antibodies, biomass, cardiolipins, cell walls, ceramides, chloroform, glucose, immune response, methanol, mice, polysaccharides, unsaturated fatty acids
The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl₃–MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C₁₈:₁ (43.6%) and C₁₈:₂ (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.