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Some biomolecules and a partially O-acetylated exo-galactomannan containing β-Galf units from pathogenic Exophiala jeanselmei, having a pronounced immunogenic response

Author:
Sassaki, G.L., Czelusniak, P.A., Vicente, V.A., Zanata, S.M., Souza, L.M., Gorin, P.A.J., Iacomini, M.
Source:
International journal of biological macromolecules 2011 v.48 no.1 pp. 177-182
ISSN:
0141-8130
Subject:
Exophiala, animal pathogenic fungi, antibodies, biomass, cardiolipins, cell walls, ceramides, chloroform, glucose, immune response, methanol, mice, polysaccharides, unsaturated fatty acids
Abstract:
The pathogenic fungus Exophiala jeanselmei (Ej4) was grown in submerged MM medium, glucose being consumed after six days with maximum biomass and EPS production. Cells were extracted with CHCl₃–MeOH (2:1, v/v) yielding a product containing 10% lipid, with high levels of unsaturated C₁₈:₁ (43.6%) and C₁₈:₂ (21.0%), 2D-TLC showed the presence of PE (17.7%), PS (11.6%), PC (35.8%), PI (1.2%) and lyso-phospholipids, LPE (10.7%), LPC (2.0%), PA (10.4%), cardiolipin (10.5%) and glucosyl-ceramide. Analysis of EPS-1 (120kDa) showed a galactomanan, containing a main chain of Manp-(1→2) (24.2%), substituted by side chains containing terminal Galf (16.8%) and Manp (3.5%) and acetyl groups attached at O-6 of terminal Galf. An immune response against antigens was obtained using Balb/C mice. Anti-EPS-1 antibodies recognized purified fraction containing cellular walls very titer and higher than 1:20,000 for EPS. The studied biomolecules showed biotechnological potential and point to important perspectives in diagnosis of fungi and immunomodulatory products.
Agid:
663806