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Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria

Author:
Moller, I.M., Roberts, T.H., Rasmusson, A.G.
Source:
Plant physiology 1996 v.112 no.1 pp. 75-78
ISSN:
0032-0889
Subject:
Solanum tuberosum, tubers, mitochondria, oxidation, ubiquinones, NADH dehydrogenase, enzyme activity, membrane potential, membrane permeability, rotenone, electron transfer, NAD (coenzyme)
Abstract:
The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.
Agid:
664455