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Cis-acting elements essential for light regulation of the nuclear gene encoding the A subunit of chloroplast glyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana

Author:
Park, S.C., Kwon, H.B., Shih, M.C.
Source:
Plant physiology 1996 v.112 no.4 pp. 1563-1571
ISSN:
0032-0889
Subject:
Arabidopsis thaliana, glyceraldehyde-3-phosphate dehydrogenase, gene expression, chromosome mapping, transcriptional activation, Nicotiana tabacum, genetic transformation, transgenic plants, complementary DNA, regulatory sequences, DNA-binding proteins, DNA-binding domains, light, repetitive sequences
Abstract:
We report the characterization of cis-acting elements involved in light regulation of the nuclear gene (GapA) that encodes the A subunit of glyceraldehyde 3-phosphate dehydrogenase in Arabidopsis thaliana. Our previous deletion analyses indicate that the -277 to -195 upstream region of GapA is essential for light induction of the beta-glucuronidase reporter gene in transgenic tobacco (Nicotiana tabacum) plants. This region contains three direct repeats with the consensus sequence 5'-CAAATGAA(A/G)A-3' (Gap boxes). Our results show that 2-bp substitutions of the last four nucleotides (AA or GA) of the Gap boxes by CC abolish light induction of the beta-glucuronidase reporter gene in vivo and affect binding of the Gap box binding factor in vitro. We have also identified an additional cis-acting element, AE (Activation Element) box, that is involved in regulation of GapA. A combination of a Gap box trimer and an AE box dimer can confer light responsiveness on the cauliflower mosaic virus 35S promoter containing the -92 to +6 upstream sequence, whereas oligomers of Gap boxes or AE boxes alone cannot confer light responsiveness on thesame promoter. These results suggest that Gap boxes and AE boxes function together as the light-responsive element of GapA.
Agid:
664551